Hybridization and wash reagents kit

xGen® Lockdown® Reagents have been optimized to deliver deep, even coverage of targets captured using xGen Lockdown Probes and Panels.

  • Achieve uniform coverage with hybridization and wash buffers optimized for use with xGen Lockdown Probes
  • Generate results quickly with a short, 4-hour hybridization protocol

xGen® Lockdown® Reagents are required for the recommended protocol for hybridization capture with xGen Lockdown Probes, Hybridization capture of DNA libraries using xGen Lockdown Probes and Reagents, v3.

xGen® Lockdown® Reagents

xGen Lockdown Reagents comprise hybridization and wash buffers that have been optimized for target enrichment using xGen Lockdown Probes and Panels. The reagents have not been tested with target capture probes from other manufacturers.

Kit contents
2X Hybridization Buffer
Hybridization Buffer Enhancer
2X Bead Wash Buffer
10X Wash Buffer I
10X Wash Buffer II
10X Wash Buffer III
10X Stringent Wash Buffer

xGen® Lockdown® Reagents

Achieve deep, uniform coverage

Figure 1. Optimal performance of xGen® Lockdown® Probes and Panels using xGen Lockdown Reagents. Target enrichment was performed using a DNA library that was created from human genomic DNA (Coriell) and enriched for a 57 kb region of the human genome. The hybridization and wash steps were performed using xGen Lockdown Reagents (IDT) or the SeqCap® EZ Hybridization and Wash Kit (NimbleGen). The enriched library was sequenced on an Illumina MiSeq® system. The data show (A) greater depth of coverage and (B) better coverage uniformity with xGen Lockdown Reagents.

Performance guarantee

All products have been functionally tested using the protocol, Hybridization capture of DNA libraries using xGen Lockdown Probes and Reagents, which is available online. Please contact IDT at xgen@idtdna.com for more information on tested protocols. Products are expected to be functional for up to 6 months after the "sell by" date listed on the tube.